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KMID : 0361719940050030319
Korean Journal of perinatology
1994 Volume.5 No. 3 p.319 ~ p.326
Production and Distribution of alpha1-Antitrypsin in Fetal Membranes and Tissues
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Abstract
Monoclonal antibodies against human ¥á1-Antitrypsin was produced by hybridizing SP2/0-Ag 14 mouse myeloma cells with spleen cells of Balb/c mouse immunized with ¥á1-Antitrypsin. Western . Western blotting analysis using purified ¥á1-Antitrypsin
and
serum proteins exhibited the specificity of the antibodies against ¥á1-Antitrypsin The distribution and localization of ¥á1-Antitrypsin in fetal tissues were examined using immunohistochemical techniques. ¥á1-Antitrypsin immunoreactivity was
detected
from the ammiotic membrane, chorionic membranes, lung, kidney, and skin tissues.
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